Commit 2f19b216 authored by Andrey Filippov's avatar Andrey Filippov

Added focus/tilt adjustment using LMA

parent bb1ed3e1
...@@ -46,6 +46,9 @@ import java.util.regex.Matcher; ...@@ -46,6 +46,9 @@ import java.util.regex.Matcher;
import java.util.regex.Pattern; import java.util.regex.Pattern;
//import FocusingField.FocusingFieldMeasurement;
//import FocusingField.MeasuredSample; //import FocusingField.MeasuredSample;
import Jama.Matrix; // Download here: http://math.nist.gov/javanumerics/jama/ import Jama.Matrix; // Download here: http://math.nist.gov/javanumerics/jama/
...@@ -799,7 +802,8 @@ if (MORE_BUTTONS) { ...@@ -799,7 +802,8 @@ if (MORE_BUTTONS) {
addButton("List qualB", panelCurvature,color_report); addButton("List qualB", panelCurvature,color_report);
addButton("List curv", panelCurvature,color_report); addButton("List curv", panelCurvature,color_report);
addButton("Show curv corr", panelCurvature,color_report); addButton("Show curv corr", panelCurvature,color_report);
addButton("Test measurement", panelCurvature,color_process); addButton("Test measurement", panelCurvature,color_debug);
addButton("Focus/Tilt LMA", panelCurvature,color_process);
add(panelCurvature); add(panelCurvature);
//panelGoniometer //panelGoniometer
...@@ -4513,6 +4517,26 @@ if (MORE_BUTTONS) { ...@@ -4513,6 +4517,26 @@ if (MORE_BUTTONS) {
FOCUSING_FIELD.testMeasurement(); FOCUSING_FIELD.testMeasurement();
return; return;
} }
/* ======================================================================== */
if (label.equals("Focus/Tilt LMA")) {
DEBUG_LEVEL=MASTER_DEBUG_LEVEL;
if (FOCUSING_FIELD==null) return;
FOCUSING_FIELD.setDebugLevel(DEBUG_LEVEL);
if (!FOCUS_MEASUREMENT_PARAMETERS.cameraIsConfigured) {
if (CAMERAS.showDialog("Configure cameras interface", 1, true)){
FOCUS_MEASUREMENT_PARAMETERS.cameraIsConfigured=true;
if (!FOCUS_MEASUREMENT_PARAMETERS.getLensSerial()) return;
// reset histories
MOTORS.clearPreFocus();
MOTORS.clearHistory();
} else {
IJ.showMessage("Error","Camera is not configured\nProcess canceled");
return;
}
}
while (adjustFocusTiltLMA());
return;
}
// //
/* ======================================================================== */ /* ======================================================================== */
if (label.equals("Show PSF")) { if (label.equals("Show PSF")) {
...@@ -8661,8 +8685,121 @@ if (MORE_BUTTONS) { ...@@ -8661,8 +8685,121 @@ if (MORE_BUTTONS) {
return; return;
} }
/* ===== Other methods ==================================================== */ /* ===== Other methods ==================================================== */
public boolean adjustFocusTiltLMA(){
// just for reporting distance old way
MOTORS.focusingHistory.optimalMotorPosition( // recalculate calibration to estimate current distance from center PSF
FOCUS_MEASUREMENT_PARAMETERS,
MOTORS.getMicronsPerStep(), //double micronsPerStep,
DEBUG_LEVEL);
// No-move measure, add to history
moveAndMaybeProbe(
true, // just move, not probe
null, // no move, just measure
MOTORS,
CAMERAS,
LENS_DISTORTION_PARAMETERS,
matchSimulatedPattern, // should not bee null - is null after grid center!!!
FOCUS_MEASUREMENT_PARAMETERS,
PATTERN_DETECT,
DISTORTION,
SIMUL,
COMPONENTS,
OTF_FILTER,
PSF_PARS,
THREADS_MAX,
UPDATE_STATUS,
MASTER_DEBUG_LEVEL,
DISTORTION.loop_debug_level);
//get measurement
FocusingField.FocusingFieldMeasurement fFMeasurement=MOTORS.getThisFFMeasurement(FOCUSING_FIELD);
// calculate z, tx, ty, m1,m2,m3
double [] zTxTyM1M2M3 = FOCUSING_FIELD.adjustLMA (fFMeasurement);
// show dialog: Apply, re-calculate, exit
int [] currentMotors=fFMeasurement.motors;
int [] newMotors=currentMotors.clone();
double [] zTxTy={Double.NaN,Double.NaN,Double.NaN};
if (zTxTyM1M2M3!=null){
newMotors[0]=(int) Math.round(zTxTyM1M2M3[3]);
newMotors[1]=(int) Math.round(zTxTyM1M2M3[4]);
newMotors[2]=(int) Math.round(zTxTyM1M2M3[5]);
zTxTy[0]=zTxTyM1M2M3[0];
zTxTy[1]=zTxTyM1M2M3[1];
zTxTy[2]=zTxTyM1M2M3[2];
}
double [] targetTilts={0.0,0.0};
double scaleMovement=1.0; // calculate automatically - reduce when close
GenericDialog gd = new GenericDialog("Adjusting focus/tilt");
gd.addNumericField("Target focus (relative to best composirte)",FOCUSING_FIELD.targetRelFocalShift,2,5,"um ("+IJ.d2s(zTxTy[0],3)+")");
gd.addNumericField("Target horizontal tilt (normally 0)",targetTilts[0],2,5,"um/mm ("+IJ.d2s(zTxTy[1],3)+")");
gd.addNumericField("Target vertical tilt (normally 0)",targetTilts[1],2,5,"um/mm ("+IJ.d2s(zTxTy[2],3)+")");
gd.addNumericField("Motor 1",newMotors[0],0,5,"steps ("+currentMotors[0]+")");
gd.addNumericField("Motor 2",newMotors[1],0,5,"steps ("+currentMotors[1]+")");
gd.addNumericField("Motor 3",newMotors[2],0,5,"steps ("+currentMotors[2]+")");
gd.addNumericField("Scale movement",scaleMovement,3,5,"x");
gd.addCheckbox("Filter samples/channels by Z",FOCUSING_FIELD.filterZ); // should be false after manual movement
gd.addCheckbox("Filter by value (leave lower than maximal fwhm used in focal scan mode)",FOCUSING_FIELD.filterByScanValue);
gd.addNumericField("Filter by value (remove samples above scaled best FWHM for channel/location)",FOCUSING_FIELD.filterByValueScale,2,5,"x");
gd.addNumericField("Z min",FOCUSING_FIELD.zMin,2,5,"um");
gd.addNumericField("Z max",FOCUSING_FIELD.zMax,2,5,"um");
gd.addNumericField("Z step",FOCUSING_FIELD.zStep,2,5,"um");
gd.addNumericField("Tilt min",FOCUSING_FIELD.tMin,2,5,"um/mm");
gd.addNumericField("Tilt max",FOCUSING_FIELD.tMax,2,5,"um/mm");
gd.addNumericField("Tilt step",FOCUSING_FIELD.tStep,2,5,"um/mm");
gd.addNumericField("Motor anti-hysteresis travel (last measured was "+IJ.d2s(FOCUS_MEASUREMENT_PARAMETERS.measuredHysteresis,0)+")", FOCUS_MEASUREMENT_PARAMETERS.motorHysteresis, 0,7,"motors steps");
gd.addNumericField("Debug Level:", MASTER_DEBUG_LEVEL, 0);
gd.enableYesNoCancel("Apply movement","Re-measure"); // default OK (on enter) - "Apply"
WindowTools.addScrollBars(gd);
gd.showDialog();
if (gd.wasCanceled()) return false;
FOCUSING_FIELD.targetRelFocalShift=gd.getNextNumber();
targetTilts[0]= gd.getNextNumber();
targetTilts[1]= gd.getNextNumber();
newMotors[0]= (int) gd.getNextNumber();
newMotors[1]= (int) gd.getNextNumber();
newMotors[2]= (int) gd.getNextNumber();
scaleMovement= gd.getNextNumber();
FOCUSING_FIELD.filterZ= gd.getNextBoolean();
FOCUSING_FIELD.filterByScanValue= gd.getNextBoolean();
FOCUSING_FIELD.filterByValueScale= gd.getNextNumber();
FOCUSING_FIELD.zMin= gd.getNextNumber();
FOCUSING_FIELD.zMax= gd.getNextNumber();
FOCUSING_FIELD.zStep= gd.getNextNumber();
FOCUSING_FIELD.tMin= gd.getNextNumber();
FOCUSING_FIELD.tMax= gd.getNextNumber();
FOCUSING_FIELD.tStep= gd.getNextNumber();
FOCUS_MEASUREMENT_PARAMETERS.motorHysteresis= (int) gd.getNextNumber();
MASTER_DEBUG_LEVEL=( int) gd.getNextNumber();
DEBUG_LEVEL=MASTER_DEBUG_LEVEL;
FOCUSING_FIELD.setDebugLevel(DEBUG_LEVEL);
// Scale motor movement
newMotors[0]=currentMotors[0]+((int) Math.round((newMotors[0]-currentMotors[0])*scaleMovement));
newMotors[1]=currentMotors[1]+((int) Math.round((newMotors[1]-currentMotors[1])*scaleMovement));
newMotors[2]=currentMotors[2]+((int) Math.round((newMotors[2]-currentMotors[2])*scaleMovement));
if (gd.wasOKed()){
// Move, no measure
MOTORS.moveElphel10364Motors(
true, //boolean wait,
newMotors,
0.0, //double sleep,
true, //boolean showStatus,
"", //String message,
false); //!noHysteresis);
}
return true;
}
public boolean checkSerialAndRestore(){ public boolean checkSerialAndRestore(){
// wait for camera // wait for camera
CAMERAS.debugLevel=DEBUG_LEVEL; CAMERAS.debugLevel=DEBUG_LEVEL;
......
...@@ -3084,12 +3084,59 @@ public class CalibrationHardwareInterface { ...@@ -3084,12 +3084,59 @@ public class CalibrationHardwareInterface {
pY0, pY0,
sampleCoord); sampleCoord);
} }
public FocusingField.FocusingFieldMeasurement getThisFFMeasurement(FocusingField focusingField){
return getThisFFMeasurement(focusingField, -1);
}
public FocusingField.FocusingFieldMeasurement getThisFFMeasurement(FocusingField focusingField, int index){
return focusingField. getFocusingFieldMeasurement(
historyGetTimestamp(index), //focusingState.getTimestamp(),
historyGetTemperature(index), //focusingState.getTemperature(),
historyGetMotors(index), //focusingState.motorsPos,
historyGetSamples(index)); //focusingState.getSamples());
}
private FocusingHistory.FocusingState getFocusingState(int index){
if (index<0) index+=historySize();
if ((index>=0) && (index<historySize())) return this.focusingHistory.history.get(index);
return null;
}
// public void addCurrentHistoryToFocusingField(FocusingField focusingField){
// this.focusingHistory.addCurrentHistory(focusingField);
// }
public void addCurrentHistoryToFocusingField(FocusingField focusingField){ public void addCurrentHistoryToFocusingField(FocusingField focusingField){
this.focusingHistory.addCurrentHistory(focusingField); for (int i=0;i<historySize();i++){
addCurrentHistoryToFocusingField(focusingField,i);
}
// this.focusingHistory.addCurrentHistory(focusingField);
}
public void addCurrentHistoryToFocusingField(
FocusingField focusingField,
int index){ // -1 - last (negative - from length)
focusingField.addSample(
historyGetTimestamp(index), //focusingState.getTimestamp(),
historyGetTemperature(index), //focusingState.getTemperature(),
historyGetMotors(index), //focusingState.motorsPos,
historyGetSamples(index)); //focusingState.getSamples());
}
public String historyGetTimestamp(int index){
return getFocusingState(index).getTimestamp();
}
public double historyGetTemperature(int index){
return getFocusingState(index).getTemperature();
}
public int [] historyGetMotors(int index){
return getFocusingState(index).getMotors();
} }
public int historySize(){ public double [][][][] historyGetSamples(int index){
return getFocusingState(index).getSamples();
}
public int historySize(){
return this.focusingHistory.history.size(); return this.focusingHistory.history.size();
} }
public void setLastProbed(){ public void setLastProbed(){
...@@ -5470,7 +5517,7 @@ if (debugLevel>=debugThreshold) System.out.println(i+" "+diff[0]+" "+diff[1]+" " ...@@ -5470,7 +5517,7 @@ if (debugLevel>=debugThreshold) System.out.println(i+" "+diff[0]+" "+diff[1]+" "
Double.NaN, Double.NaN,
Double.NaN); Double.NaN);
} }
/*
public void addCurrentHistory( public void addCurrentHistory(
FocusingField focusingField) FocusingField focusingField)
{ {
...@@ -5484,7 +5531,8 @@ if (debugLevel>=debugThreshold) System.out.println(i+" "+diff[0]+" "+diff[1]+" " ...@@ -5484,7 +5531,8 @@ if (debugLevel>=debugThreshold) System.out.println(i+" "+diff[0]+" "+diff[1]+" "
} }
} }
*/
public void saveXML( public void saveXML(
String path, String path,
String serialNumber, String serialNumber,
......
...@@ -3949,6 +3949,19 @@ public boolean LevenbergMarquardt( ...@@ -3949,6 +3949,19 @@ public boolean LevenbergMarquardt(
return nodeList; return nodeList;
} }
} }
public FocusingFieldMeasurement getFocusingFieldMeasurement(
String timestamp,
double temperature,
int [] motors,
double [][][][] samples
){
return new FocusingFieldMeasurement(
timestamp,
temperature,
motors,
samples);
}
public FocusingField( public FocusingField(
String serialNumber, String serialNumber,
String lensSerial, // if null - do not add average String lensSerial, // if null - do not add average
...@@ -4341,11 +4354,69 @@ public boolean LevenbergMarquardt( ...@@ -4341,11 +4354,69 @@ public boolean LevenbergMarquardt(
// fieldFitting.mechanicalFocusingModel.setZTxTy(0.0,0.0,0.0); // restore zeros to correctly find Z centers, // fieldFitting.mechanicalFocusingModel.setZTxTy(0.0,0.0,0.0); // restore zeros to correctly find Z centers,
fieldFitting.mechanicalFocusingModel.setAdjustMode(false); // to correctly find Z centers, fieldFitting.mechanicalFocusingModel.setAdjustMode(false); // to correctly find Z centers,
} }
//,
public double [] adjustLMA (FocusingFieldMeasurement measurement){
if (!testMeasurement(
measurement,
zMin, //+best_qb_corr[0],
zMax, //+best_qb_corr[0],
zStep,
tMin,
tMax,
tStep)) {
if (debugLevel>0) System.out.println("adjustLMA() failed");
return null;
}
double [] result=new double [6];
double [] best_qb_corr= fieldFitting.getBestQualB(
k_red,
k_blue,
true);
double [] zTilts=getCenterZTxTy(measurement);
result[0]=zTilts[0]-best_qb_corr[0];
result[1]=zTilts[1];
result[2]=zTilts[2];
double [] dm= getAdjustedMotors(
targetRelFocalShift+best_qb_corr[0],
0.0, // targetTiltX, // for testing, normally should be 0 um/mm
0.0, // targetTiltY,
true); // motor steps
if ((dm!=null) && (debugLevel>1)){
System.out.println("Suggested motor positions: "+IJ.d2s(dm[0],0)+":"+IJ.d2s(dm[1],0)+":"+IJ.d2s(dm[2],0));
}
if (dm!=null) {
result[3]=dm[0];
result[4]=dm[1];
result[5]=dm[2];
} else {
result[3]=Double.NaN;
result[4]=Double.NaN;
result[5]=Double.NaN;
}
return result;
}
// add tx, ty?
public double[] getCenterZTxTy(FocusingFieldMeasurement measurement){
double [] tilts= fieldFitting.mechanicalFocusingModel.getTilts(measurement.motors);
double [] result = {
fieldFitting.mechanicalFocusingModel.calc_ZdZ(
measurement.motors,
currentPX0, //fieldFitting.getCenterXY()[0],
currentPY0, //fieldFitting.getCenterXY()[1],
null),
tilts[0],
tilts[1]
};
return result;
}
public double [] getAdjustedMotors( public double [] getAdjustedMotors(
double targetRelFocalShift, double targetRelFocalShift,
double targetTiltX, // for testing, normally should be 0 um/mm double targetTiltX, // for testing, normally should be 0 um/mm
double targetTiltY, double targetTiltY, // for testing, normally should be 0 um/mm
boolean motorSteps){ // for testing, normally should be 0 um/mm boolean motorSteps){
double [] zM=fieldFitting.mechanicalFocusingModel.getZM( double [] zM=fieldFitting.mechanicalFocusingModel.getZM(
currentPX0, //fieldFitting.getCenterXY()[0], currentPX0, //fieldFitting.getCenterXY()[0],
currentPY0, //fieldFitting.getCenterXY()[1], currentPY0, //fieldFitting.getCenterXY()[1],
...@@ -6370,6 +6441,25 @@ public boolean LevenbergMarquardt( ...@@ -6370,6 +6441,25 @@ public boolean LevenbergMarquardt(
} }
return z; return z;
} }
public double [] getTilts(int [] motors){
double kM1= getValue(MECH_PAR.K0)+getValue(MECH_PAR.KD1)-getValue(MECH_PAR.KD3);
double kM2= getValue(MECH_PAR.K0)-getValue(MECH_PAR.KD1)-getValue(MECH_PAR.KD3);
double kM3= getValue(MECH_PAR.K0)+getValue(MECH_PAR.KD3);
double p2pi= PERIOD/2/Math.PI;
double m1=motors[0],m2=motors[1],m3=motors[2];
double aM1=(m1 + getValue(MECH_PAR.sM1)*p2pi*Math.sin(m1/p2pi) + getValue(MECH_PAR.cM1)*p2pi*Math.cos(m1/p2pi));
double aM2=(m2 + getValue(MECH_PAR.sM2)*p2pi*Math.sin(m2/p2pi) + getValue(MECH_PAR.cM2)*p2pi*Math.cos(m2/p2pi));
double aM3=(m3 + getValue(MECH_PAR.sM3)*p2pi*Math.sin(m3/p2pi) + getValue(MECH_PAR.cM3)*p2pi*Math.cos(m3/p2pi));
double zM1=kM1 * aM1;
double zM2=kM2 * aM2;
double zM3=kM3 * aM3;
double [] result ={
getValue(MECH_PAR.tx)+(2*zM3-zM1-zM2)/(4*getValue(MECH_PAR.Lx)),
getValue(MECH_PAR.ty)-(zM2-zM1)/(2*getValue(MECH_PAR.Ly))};
return result;
}
/** /**
* Calculate linearized mount (motor) displacement from motor position in steps * Calculate linearized mount (motor) displacement from motor position in steps
...@@ -6522,9 +6612,9 @@ public boolean LevenbergMarquardt( ...@@ -6522,9 +6612,9 @@ public boolean LevenbergMarquardt(
} }
}; };
double [][] B={ double [][] B={
{targetZ-getValue(MECH_PAR.z0)}, {targetZ-getValue(MECH_PAR.z0)}, // calc_ZdZ()?
{targetTx-dx*getValue(MECH_PAR.tx)}, {targetTx-getValue(MECH_PAR.tx)},
{targetTy-dy*getValue(MECH_PAR.ty)} {targetTy-getValue(MECH_PAR.ty)}
}; };
Matrix MA=new Matrix(A); Matrix MA=new Matrix(A);
Matrix MB=new Matrix(B); Matrix MB=new Matrix(B);
......
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