Loading imagej_tiffwriter_test.py +72 −236 Original line number Original line Diff line number Diff line #!/usr/bin/env python3 #!/usr/bin/env python3 ''' import struct /** import numpy * @file imagej_tiff_saver.py import tifffile * @brief save tiffs for imagej (1.52d+) - with stacks and hyperstacks * @par <b>License</b>: * This program is free software: you can redistribute it and/or modify * it under the terms of the GNU General Public License as published by * the Free Software Foundation, either version 3 of the License, or * (at your option) any later version. * * This program is distributed in the hope that it will be useful, * but WITHOUT ANY WARRANTY; without even the implied warranty of * MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the * GNU General Public License for more details. * * You should have received a copy of the GNU General Public License * along with this program. If not, see <http://www.gnu.org/licenses/>. */ ''' __copyright__ = "Copyright 2018, Elphel, Inc." __license__ = "GPL-3.0+" __email__ = "oleg@elphel.com" ''' Usage example: import imagej_tiffwriter import numpy as np # have a few images in the form of numpy arrays # make sure to stack them as: # - (t,z,h,w,c) # - (z,h,w,c) # - (h,w,c) # - (h,w) imagej_tiffwriter.save(path,images) ''' from PIL import Image, TiffImagePlugin import numpy as np import math import math # DO NOT USE? def imagej_metadata_tags(metadata, byteorder): # thing is the old ImageJs <1.52d poorly handle tags directories or something like that """Return IJMetadata and IJMetadataByteCounts tags from metadata dict. def __get_IJ_IFD(t,z,c): The tags can be passed to the TiffWriter.save function as extratags. #ifd = TiffImagePlugin.ImageFileDirectory_v2(ifh=b"MM*\x00\x00\x00\x00\x00",prefix= b"MM") ifd = TiffImagePlugin.ImageFileDirectory_v2(prefix= b"MM") """ #ifd = TiffImagePlugin.ImageFileDirectory_v2() header = [{'>': b'IJIJ', '<': b'JIJI'}[byteorder]] #ifd = TiffImagePlugin.ImageFileDirectory(prefix='MM') bytecounts = [0] body = [] ijheader = [ 'ImageJ=', def writestring(data, byteorder): 'hyperstack=true', return data.encode('utf-16' + {'>': 'be', '<': 'le'}[byteorder]) 'images='+str(t*z*c), 'channels='+str(c), def writedoubles(data, byteorder): 'slices='+str(z), return struct.pack(byteorder+('d' * len(data)), *data) 'frames='+str(t), 'loop=false' def writebytes(data, byteorder): ] return data.tobytes() ifd[270] = ("\n".join(ijheader)+"\n") metadata_types = ( ('Info', b'info', 1, writestring), ijlabl = [ ('Labels', b'labl', None, writestring), b'IJIJinfo\x00\x00\x00\x01labl\x00\x00\x00\x05', ('Ranges', b'rang', 1, writedoubles), '<info></info>'.encode('UTF-16-LE'), ('LUTs', b'luts', None, writebytes), 'img1'.encode('UTF-16-LE'), ('Plot', b'plot', 1, writebytes), 'img2'.encode('UTF-16-LE'), ('ROI', b'roi ', 1, writebytes), 'img3'.encode('UTF-16-LE'), ('Overlays', b'over', None, writebytes)) 'img4'.encode('UTF-16-LE'), 'img5'.encode('UTF-16-LE') for key, mtype, count, func in metadata_types: ] if key not in metadata: continue #for i in range() if byteorder == '<': mtype = mtype[::-1] #ifd[50838] = (20,10) values = metadata[key] #ifd[50839] = (20,10) if count is None: ifd_50838_list = [] count = len(values) ifd_50839 = b"".join(ijlabl) for label in ijlabl: ifd_50838_list.append(len(label)) ifd_50838 = tuple(ifd_50838_list) #print(ifd_50838) #print(ifd_50839) #is_hyperstack = 'true' if len(shape)>1 else 'false' #if (len(shape)>0): ifd[50838] = ifd_50838 ifd[50839] = ifd_50839 # override #tif = Image.open("test.tiff") #tag_50838 = tif.tag[50838] #tag_50839 = tif.tag[50839] #tag_270 = tif.tag[270] #print(tag_50839) #ifd[270] = tag_270 #ifd[50838] = tag_50838 #ifd[50839] = tag_50839 return ifd #def save(path,images,force_stack=False,force_hyperstack=False): def save(path,images): # Got images, analyze shape: # - possible formats (c == depth): # -- (t,z,h,w,c) # -- (t,h,w,c), t or z does not matter # -- (h,w,c) # -- (h,w) # 0 or 1 images.shapes are not handled # # (h,w) if len(images.shape)==2: image = Image.fromarray(images) image.save(path) elif len(images.shape)>2: h,w,c = images.shape[-3:] if len(images.shape)==3: images = np.reshape(images,(1,h,w,c)) z = images.shape[-4] if len(images.shape)==4: images = np.reshape(images,(1,z,h,w,c)) t = images.shape[-5] c_axis = -1 if c==1: split_channels = images else: else: channels = np.array(np.split(images,c,axis=c_axis)) values = [values] split_channels = np.concatenate(channels,axis=-3) header.append(mtype + struct.pack(byteorder+'I', count)) for value in values: images_flat = np.reshape(split_channels,(-1,h,w)) data = func(value, byteorder) body.append(data) imlist = [] bytecounts.append(len(data)) for i in range(images_flat.shape[0]): imlist.append(Image.fromarray(images_flat[i])) body = b''.join(body) header = b''.join(header) data = header + body bytecounts[0] = len(header) bytecounts = struct.pack(byteorder+('I' * len(bytecounts)), *bytecounts) return ((50839, 'B', len(data), data, True), (50838, 'I', len(bytecounts)//4, bytecounts, True)) #imlist[0].save(path,save_all=True,append_images=imlist[1:]) # thing is the old ImageJs <1.52d poorly handle tags directories or something like that imlist[0].save(path,save_all=True,append_images=imlist[1:],tiffinfo=__get_IJ_IFD(t,z,c)) # Testing if __name__ == "__main__": def hamming_window(x,N): def hamming_window(x,N): y = 0.54 - 0.46*math.cos(2*math.pi*x/(N-1)) y = 0.54 - 0.46*math.cos(2*math.pi*x/(N-1)) Loading @@ -170,71 +64,13 @@ if __name__ == "__main__": hw = hamming_window hw = hamming_window NT = 5 image = numpy.array([[(255-0*25)*hw(i,512)*hw(j,512) for i in range(512)] for j in range(512)],numpy.float32) NC = 1 image = image[numpy.newaxis,...] NZ = 1 NX = 2916 NY = 2178 images = np.empty((NT,NZ,NY,NX,NC)) import time print(str(time.time())+": Generating test images") for t in range(NT): for z in range(NZ): for c in range(NC): images[t,z,:,:,c] = np.array([[(255-t*25)*hw(i,512)*hw(j,512) for i in range(NX)] for j in range(NY)],np.float32) print(str(time.time())+": Test images generated") print("Images shape: "+str(images.shape)) print("1D run") imgs = images[:,0,:,:,0] print(imgs.shape) imlist = [] for i in range(imgs.shape[0]): tmp = Image.fromarray(imgs[i]) #tmp.mode = "I;32BS" imlist.append(tmp) TiffImagePlugin.DEBUG = True TiffImagePlugin.WRITE_LIBTIFF = False #import sys #sys.byteorder = "big" #imlist[0].mode = "I;16B" #print("encoderconfig") #print(imlist[0].encoderconfig) imlist[0].save("result_1D.tiff",save_all=True,append_images=imlist[1:],tiffinfo=__get_IJ_IFD(NT,NZ,NC)) #v = save("result_1D.tiff",imgs) #tif = Image.open("result_1D.tiff") #tag_50838 = tif.tag[50838] #tag_50839 = tif.tag[50839] #tag_270 = tif.tag[270] #print(tag_50839) #tif = Image.open("test.tiff") #tif.save("test_saved.tiff") #print("5D run") #v = save("result_5D.tiff",images) #print("4D run") #v = save("result_4D.tiff",images[0]) #print("3D run") #v = save("result_3D.tiff",images[0,0]) #print("3D run, 1 channel") #tmp_images = images[0,0,:,:,0] #v = save("result_3D1C.tiff",tmp_images[:,:,np.newaxis]) # open test ijtags = imagej_metadata_tags({'Labels':["Name1","Name2","Name3","Name4","Name5"]}, '<') print(ijtags) with tifffile.TiffWriter("multipage_test.tiff", bigtiff=False,imagej=True) as tif: for i in range(5): tif.save(image, metadata={'version':'20180905', 'loop':False}, extratags=ijtags) Loading
imagej_tiffwriter_test.py +72 −236 Original line number Original line Diff line number Diff line #!/usr/bin/env python3 #!/usr/bin/env python3 ''' import struct /** import numpy * @file imagej_tiff_saver.py import tifffile * @brief save tiffs for imagej (1.52d+) - with stacks and hyperstacks * @par <b>License</b>: * This program is free software: you can redistribute it and/or modify * it under the terms of the GNU General Public License as published by * the Free Software Foundation, either version 3 of the License, or * (at your option) any later version. * * This program is distributed in the hope that it will be useful, * but WITHOUT ANY WARRANTY; without even the implied warranty of * MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the * GNU General Public License for more details. * * You should have received a copy of the GNU General Public License * along with this program. If not, see <http://www.gnu.org/licenses/>. */ ''' __copyright__ = "Copyright 2018, Elphel, Inc." __license__ = "GPL-3.0+" __email__ = "oleg@elphel.com" ''' Usage example: import imagej_tiffwriter import numpy as np # have a few images in the form of numpy arrays # make sure to stack them as: # - (t,z,h,w,c) # - (z,h,w,c) # - (h,w,c) # - (h,w) imagej_tiffwriter.save(path,images) ''' from PIL import Image, TiffImagePlugin import numpy as np import math import math # DO NOT USE? def imagej_metadata_tags(metadata, byteorder): # thing is the old ImageJs <1.52d poorly handle tags directories or something like that """Return IJMetadata and IJMetadataByteCounts tags from metadata dict. def __get_IJ_IFD(t,z,c): The tags can be passed to the TiffWriter.save function as extratags. #ifd = TiffImagePlugin.ImageFileDirectory_v2(ifh=b"MM*\x00\x00\x00\x00\x00",prefix= b"MM") ifd = TiffImagePlugin.ImageFileDirectory_v2(prefix= b"MM") """ #ifd = TiffImagePlugin.ImageFileDirectory_v2() header = [{'>': b'IJIJ', '<': b'JIJI'}[byteorder]] #ifd = TiffImagePlugin.ImageFileDirectory(prefix='MM') bytecounts = [0] body = [] ijheader = [ 'ImageJ=', def writestring(data, byteorder): 'hyperstack=true', return data.encode('utf-16' + {'>': 'be', '<': 'le'}[byteorder]) 'images='+str(t*z*c), 'channels='+str(c), def writedoubles(data, byteorder): 'slices='+str(z), return struct.pack(byteorder+('d' * len(data)), *data) 'frames='+str(t), 'loop=false' def writebytes(data, byteorder): ] return data.tobytes() ifd[270] = ("\n".join(ijheader)+"\n") metadata_types = ( ('Info', b'info', 1, writestring), ijlabl = [ ('Labels', b'labl', None, writestring), b'IJIJinfo\x00\x00\x00\x01labl\x00\x00\x00\x05', ('Ranges', b'rang', 1, writedoubles), '<info></info>'.encode('UTF-16-LE'), ('LUTs', b'luts', None, writebytes), 'img1'.encode('UTF-16-LE'), ('Plot', b'plot', 1, writebytes), 'img2'.encode('UTF-16-LE'), ('ROI', b'roi ', 1, writebytes), 'img3'.encode('UTF-16-LE'), ('Overlays', b'over', None, writebytes)) 'img4'.encode('UTF-16-LE'), 'img5'.encode('UTF-16-LE') for key, mtype, count, func in metadata_types: ] if key not in metadata: continue #for i in range() if byteorder == '<': mtype = mtype[::-1] #ifd[50838] = (20,10) values = metadata[key] #ifd[50839] = (20,10) if count is None: ifd_50838_list = [] count = len(values) ifd_50839 = b"".join(ijlabl) for label in ijlabl: ifd_50838_list.append(len(label)) ifd_50838 = tuple(ifd_50838_list) #print(ifd_50838) #print(ifd_50839) #is_hyperstack = 'true' if len(shape)>1 else 'false' #if (len(shape)>0): ifd[50838] = ifd_50838 ifd[50839] = ifd_50839 # override #tif = Image.open("test.tiff") #tag_50838 = tif.tag[50838] #tag_50839 = tif.tag[50839] #tag_270 = tif.tag[270] #print(tag_50839) #ifd[270] = tag_270 #ifd[50838] = tag_50838 #ifd[50839] = tag_50839 return ifd #def save(path,images,force_stack=False,force_hyperstack=False): def save(path,images): # Got images, analyze shape: # - possible formats (c == depth): # -- (t,z,h,w,c) # -- (t,h,w,c), t or z does not matter # -- (h,w,c) # -- (h,w) # 0 or 1 images.shapes are not handled # # (h,w) if len(images.shape)==2: image = Image.fromarray(images) image.save(path) elif len(images.shape)>2: h,w,c = images.shape[-3:] if len(images.shape)==3: images = np.reshape(images,(1,h,w,c)) z = images.shape[-4] if len(images.shape)==4: images = np.reshape(images,(1,z,h,w,c)) t = images.shape[-5] c_axis = -1 if c==1: split_channels = images else: else: channels = np.array(np.split(images,c,axis=c_axis)) values = [values] split_channels = np.concatenate(channels,axis=-3) header.append(mtype + struct.pack(byteorder+'I', count)) for value in values: images_flat = np.reshape(split_channels,(-1,h,w)) data = func(value, byteorder) body.append(data) imlist = [] bytecounts.append(len(data)) for i in range(images_flat.shape[0]): imlist.append(Image.fromarray(images_flat[i])) body = b''.join(body) header = b''.join(header) data = header + body bytecounts[0] = len(header) bytecounts = struct.pack(byteorder+('I' * len(bytecounts)), *bytecounts) return ((50839, 'B', len(data), data, True), (50838, 'I', len(bytecounts)//4, bytecounts, True)) #imlist[0].save(path,save_all=True,append_images=imlist[1:]) # thing is the old ImageJs <1.52d poorly handle tags directories or something like that imlist[0].save(path,save_all=True,append_images=imlist[1:],tiffinfo=__get_IJ_IFD(t,z,c)) # Testing if __name__ == "__main__": def hamming_window(x,N): def hamming_window(x,N): y = 0.54 - 0.46*math.cos(2*math.pi*x/(N-1)) y = 0.54 - 0.46*math.cos(2*math.pi*x/(N-1)) Loading @@ -170,71 +64,13 @@ if __name__ == "__main__": hw = hamming_window hw = hamming_window NT = 5 image = numpy.array([[(255-0*25)*hw(i,512)*hw(j,512) for i in range(512)] for j in range(512)],numpy.float32) NC = 1 image = image[numpy.newaxis,...] NZ = 1 NX = 2916 NY = 2178 images = np.empty((NT,NZ,NY,NX,NC)) import time print(str(time.time())+": Generating test images") for t in range(NT): for z in range(NZ): for c in range(NC): images[t,z,:,:,c] = np.array([[(255-t*25)*hw(i,512)*hw(j,512) for i in range(NX)] for j in range(NY)],np.float32) print(str(time.time())+": Test images generated") print("Images shape: "+str(images.shape)) print("1D run") imgs = images[:,0,:,:,0] print(imgs.shape) imlist = [] for i in range(imgs.shape[0]): tmp = Image.fromarray(imgs[i]) #tmp.mode = "I;32BS" imlist.append(tmp) TiffImagePlugin.DEBUG = True TiffImagePlugin.WRITE_LIBTIFF = False #import sys #sys.byteorder = "big" #imlist[0].mode = "I;16B" #print("encoderconfig") #print(imlist[0].encoderconfig) imlist[0].save("result_1D.tiff",save_all=True,append_images=imlist[1:],tiffinfo=__get_IJ_IFD(NT,NZ,NC)) #v = save("result_1D.tiff",imgs) #tif = Image.open("result_1D.tiff") #tag_50838 = tif.tag[50838] #tag_50839 = tif.tag[50839] #tag_270 = tif.tag[270] #print(tag_50839) #tif = Image.open("test.tiff") #tif.save("test_saved.tiff") #print("5D run") #v = save("result_5D.tiff",images) #print("4D run") #v = save("result_4D.tiff",images[0]) #print("3D run") #v = save("result_3D.tiff",images[0,0]) #print("3D run, 1 channel") #tmp_images = images[0,0,:,:,0] #v = save("result_3D1C.tiff",tmp_images[:,:,np.newaxis]) # open test ijtags = imagej_metadata_tags({'Labels':["Name1","Name2","Name3","Name4","Name5"]}, '<') print(ijtags) with tifffile.TiffWriter("multipage_test.tiff", bigtiff=False,imagej=True) as tif: for i in range(5): tif.save(image, metadata={'version':'20180905', 'loop':False}, extratags=ijtags)