Loading imagej_tiffwriter.py +86 −77 Original line number Diff line number Diff line Loading @@ -40,36 +40,77 @@ Usage example: ''' from PIL import Image, TiffImagePlugin import numpy as np import struct import tifffile import math # DO NOT USE? # thing is the old ImageJs <1.52d poorly handle tags directories or something like that def __get_IJ_IFD(t,z,c): ifd = TiffImagePlugin.ImageFileDirectory_v2() ijheader = [ 'ImageJ=', 'hyperstack=true', 'images='+str(t*z*c), 'channels='+str(c), 'slices='+str(z), 'frames='+str(t), 'loop=false' ] ifd[270] = ("\n".join(ijheader)+"\n") # from here: https://stackoverflow.com/questions/50258287/how-to-specify-colormap-when-saving-tiff-stack def imagej_metadata_tags(metadata, byteorder): """Return IJMetadata and IJMetadataByteCounts tags from metadata dict. The tags can be passed to the TiffWriter.save function as extratags. """ header = [{'>': b'IJIJ', '<': b'JIJI'}[byteorder]] bytecounts = [0] body = [] def writestring(data, byteorder): return data.encode('utf-16' + {'>': 'be', '<': 'le'}[byteorder]) def writedoubles(data, byteorder): return struct.pack(byteorder+('d' * len(data)), *data) def writebytes(data, byteorder): return data.tobytes() metadata_types = ( ('Info', b'info', 1, writestring), ('Labels', b'labl', None, writestring), ('Ranges', b'rang', 1, writedoubles), ('LUTs', b'luts', None, writebytes), ('Plot', b'plot', 1, writebytes), ('ROI', b'roi ', 1, writebytes), ('Overlays', b'over', None, writebytes)) for key, mtype, count, func in metadata_types: if key not in metadata: continue if byteorder == '<': mtype = mtype[::-1] values = metadata[key] if count is None: count = len(values) else: values = [values] header.append(mtype + struct.pack(byteorder+'I', count)) for value in values: data = func(value, byteorder) body.append(data) bytecounts.append(len(data)) body = b''.join(body) header = b''.join(header) data = header + body bytecounts[0] = len(header) bytecounts = struct.pack(byteorder+('I' * len(bytecounts)), *bytecounts) return ((50839, 'B', len(data), data, True), (50838, 'I', len(bytecounts)//4, bytecounts, True)) #is_hyperstack = 'true' if len(shape)>1 else 'false' #if (len(shape)>0): return ifd #def save(path,images,force_stack=False,force_hyperstack=False): def save(path,images,labels=None,label_prefix="Label"): ''' labels a list or None ''' #def save(path,images,force_stack=False,force_hyperstack=False): def save(path,images): ''' Expecting: (h,w), (n,h,w) - just create a simple stack ''' # Got images, analyze shape: # - possible formats (c == depth): Loading @@ -78,46 +119,33 @@ def save(path,images): # -- (h,w,c) # -- (h,w) # 0 or 1 images.shapes are not handled # # (h,w) if len(images.shape)==2: images = images[np.newaxis,...] image = Image.fromarray(images) image.save(path) elif len(images.shape)>2: h,w,c = images.shape[-3:] # now the shape length is 3 if len(images.shape)==3: images = np.reshape(images,(1,h,w,c)) z = images.shape[-4] if len(images.shape)==4: images = np.reshape(images,(1,z,h,w,c)) t = images.shape[-5] c_axis = -1 if c==1: split_channels = images # tifffile treats shape[0] as channel, need to expand to get labels displayed #images = images[images.shape[0],np.newaxis,images.shape[1],images.shape[2]] images = np.reshape(images,(images.shape[0],1,images.shape[1],images.shape[2])) labels_list = [] if labels is None: for i in range(images.shape[0]): labels_list.append(label_prefix+str(i)) else: channels = np.array(np.split(images,c,axis=c_axis)) split_channels = np.concatenate(channels,axis=-3) labels_list = labels images_flat = np.reshape(split_channels,(-1,h,w)) print(labels_list) imlist = [] for i in range(images_flat.shape[0]): imlist.append(Image.fromarray(images_flat[i])) ijtags = imagej_metadata_tags({'Labels':labels_list}, '<') imlist[0].save(path,save_all=True,append_images=imlist[1:]) # thing is the old ImageJs <1.52d poorly handle tags directories or something like that #imlist[0].save(path,save_all=True,append_images=imlist[1:],tiffinfo=__get_IJ_IFD(t,z,c)) with tifffile.TiffWriter(path, bigtiff=False,imagej=True) as tif: for i in range(images.shape[0]): print(images[i].shape) tif.save(images[i], metadata={'version':'1.11a',' loop':False}, extratags=ijtags) # Testing if __name__ == "__main__": Loading @@ -129,34 +157,15 @@ if __name__ == "__main__": hw = hamming_window NT = 5 NC = 2 NZ = 3 NX = 512 NY = 512 images = np.empty((NT,NZ,NY,NX,NC)) images = np.empty((NT,NY,NX)) import time print(str(time.time())+": Generating test images") for t in range(NT): for z in range(NZ): for c in range(NC): images[t,z,:,:,c] = np.array([[(255-t*25)*hw(i,512)*hw(j,512) for i in range(NX)] for j in range(NY)],np.float32) images[t,:,:] = np.array([[(255-t*25)*hw(i,512)*hw(j,512) for i in range(NX)] for j in range(NY)],np.float32) print(str(time.time())+": Test images generated") print("Images shape: "+str(images.shape)) print("5D run") v = save("result_5D.tiff",images) print("4D run") v = save("result_4D.tiff",images[0]) print("3D run") v = save("result_3D.tiff",images[0,0]) print("3D run, 1 channel") tmp_images = images[0,0,:,:,0] v = save("result_3D1C.tiff",tmp_images[:,:,np.newaxis]) v = save("tiffwriter_test.tiff",images) imagej_tiffwriter_test.py +0 −2 Original line number Diff line number Diff line Loading @@ -69,8 +69,6 @@ image = image[numpy.newaxis,...] ijtags = imagej_metadata_tags({'Labels':["Name1","Name2","Name3","Name4","Name5"]}, '<') print(ijtags) with tifffile.TiffWriter("multipage_test.tiff", bigtiff=False,imagej=True) as tif: for i in range(5): tif.save(image, metadata={'version':'20180905', 'loop':False}, extratags=ijtags) Loading
imagej_tiffwriter.py +86 −77 Original line number Diff line number Diff line Loading @@ -40,36 +40,77 @@ Usage example: ''' from PIL import Image, TiffImagePlugin import numpy as np import struct import tifffile import math # DO NOT USE? # thing is the old ImageJs <1.52d poorly handle tags directories or something like that def __get_IJ_IFD(t,z,c): ifd = TiffImagePlugin.ImageFileDirectory_v2() ijheader = [ 'ImageJ=', 'hyperstack=true', 'images='+str(t*z*c), 'channels='+str(c), 'slices='+str(z), 'frames='+str(t), 'loop=false' ] ifd[270] = ("\n".join(ijheader)+"\n") # from here: https://stackoverflow.com/questions/50258287/how-to-specify-colormap-when-saving-tiff-stack def imagej_metadata_tags(metadata, byteorder): """Return IJMetadata and IJMetadataByteCounts tags from metadata dict. The tags can be passed to the TiffWriter.save function as extratags. """ header = [{'>': b'IJIJ', '<': b'JIJI'}[byteorder]] bytecounts = [0] body = [] def writestring(data, byteorder): return data.encode('utf-16' + {'>': 'be', '<': 'le'}[byteorder]) def writedoubles(data, byteorder): return struct.pack(byteorder+('d' * len(data)), *data) def writebytes(data, byteorder): return data.tobytes() metadata_types = ( ('Info', b'info', 1, writestring), ('Labels', b'labl', None, writestring), ('Ranges', b'rang', 1, writedoubles), ('LUTs', b'luts', None, writebytes), ('Plot', b'plot', 1, writebytes), ('ROI', b'roi ', 1, writebytes), ('Overlays', b'over', None, writebytes)) for key, mtype, count, func in metadata_types: if key not in metadata: continue if byteorder == '<': mtype = mtype[::-1] values = metadata[key] if count is None: count = len(values) else: values = [values] header.append(mtype + struct.pack(byteorder+'I', count)) for value in values: data = func(value, byteorder) body.append(data) bytecounts.append(len(data)) body = b''.join(body) header = b''.join(header) data = header + body bytecounts[0] = len(header) bytecounts = struct.pack(byteorder+('I' * len(bytecounts)), *bytecounts) return ((50839, 'B', len(data), data, True), (50838, 'I', len(bytecounts)//4, bytecounts, True)) #is_hyperstack = 'true' if len(shape)>1 else 'false' #if (len(shape)>0): return ifd #def save(path,images,force_stack=False,force_hyperstack=False): def save(path,images,labels=None,label_prefix="Label"): ''' labels a list or None ''' #def save(path,images,force_stack=False,force_hyperstack=False): def save(path,images): ''' Expecting: (h,w), (n,h,w) - just create a simple stack ''' # Got images, analyze shape: # - possible formats (c == depth): Loading @@ -78,46 +119,33 @@ def save(path,images): # -- (h,w,c) # -- (h,w) # 0 or 1 images.shapes are not handled # # (h,w) if len(images.shape)==2: images = images[np.newaxis,...] image = Image.fromarray(images) image.save(path) elif len(images.shape)>2: h,w,c = images.shape[-3:] # now the shape length is 3 if len(images.shape)==3: images = np.reshape(images,(1,h,w,c)) z = images.shape[-4] if len(images.shape)==4: images = np.reshape(images,(1,z,h,w,c)) t = images.shape[-5] c_axis = -1 if c==1: split_channels = images # tifffile treats shape[0] as channel, need to expand to get labels displayed #images = images[images.shape[0],np.newaxis,images.shape[1],images.shape[2]] images = np.reshape(images,(images.shape[0],1,images.shape[1],images.shape[2])) labels_list = [] if labels is None: for i in range(images.shape[0]): labels_list.append(label_prefix+str(i)) else: channels = np.array(np.split(images,c,axis=c_axis)) split_channels = np.concatenate(channels,axis=-3) labels_list = labels images_flat = np.reshape(split_channels,(-1,h,w)) print(labels_list) imlist = [] for i in range(images_flat.shape[0]): imlist.append(Image.fromarray(images_flat[i])) ijtags = imagej_metadata_tags({'Labels':labels_list}, '<') imlist[0].save(path,save_all=True,append_images=imlist[1:]) # thing is the old ImageJs <1.52d poorly handle tags directories or something like that #imlist[0].save(path,save_all=True,append_images=imlist[1:],tiffinfo=__get_IJ_IFD(t,z,c)) with tifffile.TiffWriter(path, bigtiff=False,imagej=True) as tif: for i in range(images.shape[0]): print(images[i].shape) tif.save(images[i], metadata={'version':'1.11a',' loop':False}, extratags=ijtags) # Testing if __name__ == "__main__": Loading @@ -129,34 +157,15 @@ if __name__ == "__main__": hw = hamming_window NT = 5 NC = 2 NZ = 3 NX = 512 NY = 512 images = np.empty((NT,NZ,NY,NX,NC)) images = np.empty((NT,NY,NX)) import time print(str(time.time())+": Generating test images") for t in range(NT): for z in range(NZ): for c in range(NC): images[t,z,:,:,c] = np.array([[(255-t*25)*hw(i,512)*hw(j,512) for i in range(NX)] for j in range(NY)],np.float32) images[t,:,:] = np.array([[(255-t*25)*hw(i,512)*hw(j,512) for i in range(NX)] for j in range(NY)],np.float32) print(str(time.time())+": Test images generated") print("Images shape: "+str(images.shape)) print("5D run") v = save("result_5D.tiff",images) print("4D run") v = save("result_4D.tiff",images[0]) print("3D run") v = save("result_3D.tiff",images[0,0]) print("3D run, 1 channel") tmp_images = images[0,0,:,:,0] v = save("result_3D1C.tiff",tmp_images[:,:,np.newaxis]) v = save("tiffwriter_test.tiff",images)
imagej_tiffwriter_test.py +0 −2 Original line number Diff line number Diff line Loading @@ -69,8 +69,6 @@ image = image[numpy.newaxis,...] ijtags = imagej_metadata_tags({'Labels':["Name1","Name2","Name3","Name4","Name5"]}, '<') print(ijtags) with tifffile.TiffWriter("multipage_test.tiff", bigtiff=False,imagej=True) as tif: for i in range(5): tif.save(image, metadata={'version':'20180905', 'loop':False}, extratags=ijtags)